The Beer-Lambert Law is A = ELC, where A is the absorbance, E is the molar extinction coefficient, L is the path length in cm, and C is the concentration.
The Beer-Lambert Law can be expressed in the form of y = mx + b, where A is y, and C is x. What is b and how is it used to calculate the final absorbance?
b is the absorbance of another protein and is added to the absorbance of the other A values.
b is the absorbance of contaminations and is subtracted from the absorbance of the other A values.
b is the absorbance of the blank and is subtracted from the absorbance of the other A values.
b is the absorbance of the initial pure protein and is added to the absorbance of the other A values.