The $$\Delta G^{\circ'}$$ for the hydrolysis of acetyl-CoA to acetate and CoA is $$-32.2$$ kJ/mol. The $$\Delta G^{\circ'}$$ for the hydrolysis of ATP to AMP and $$PP_i$$ is $$-30.5$$ kJ/mol. Calculate $$\Delta G^{\circ'}$$ for the ATP-dependent synthesis of acetyl-CoA. $$\Delta G^{\circ'} =$$ kJ/mol Almost all cells contain the enzyme inorganic pyrophosphatase, which catalyzes the hydrolysis of $$PP_i$$ to $$P_i$$.
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The problem provides two reactions and their $$\Delta G^{\circ'}$$ values: 1. Hydrolysis of acetyl-CoA: Acetyl-CoA + $$H_2O \rightarrow$$ Acetate + CoA $$\Delta G^{\circ'}_1 = -32.2$$ kJ/mol 2. Hydrolysis of ATP to AMP and $$PP_i$$: ATP + $$H_2O Show more…
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Synthesis of the activated form of acetate (acetyl-CoA) is carried out in an ATP-dependent process: $$\text { Acetate }+\mathrm{CoA}+\mathrm{ATP} \rightarrow \text { acetyl-CoA }+\mathrm{AMP}+\mathrm{PP}_{1}$$ (a) The $\Delta G^{\prime \circ}$ for hydrolysis of acetyl-CoA to acetate and $\mathrm{CoA}$ is $-32.2 \mathrm{kJ} / \mathrm{mol}$ and that for hydrolysis of ATP to AMP and PP $_{i}$ is -30.5 kJ/mol. Calculate $\Delta G^{\prime \circ}$ for the ATPdependent synthesis of acetyl-CoA. (b) Almost all cells contain the enzyme inorganic pyrophosphatase, which catalyzes the hydrolysis of $\mathrm{PP}_{\mathrm{i}}$ to $\mathrm{P}_{\mathrm{i}}$. What effect does the presence of this enzyme have on the synthesis of acctyl-CoA? Explain.
The synthesis of acetyl CoA from acetate is an ATP-dependent reaction: Acetate + ATP + CoA <--------> Acetyl CoA + AMP + PPi a. The ΔG° for hydrolysis of acetyl CoA to acetate and CoA is -32.2 kJ/mol and that for the hydrolysis of ATP to AMP and PPi is -30 kJ/mol. Calculate the ΔG° for ATP-dependent synthesis of acetyl CoA (is the reaction spontaneous?). b. The PPi formed in the above reaction is rapidly hydrolyzed (ΔG° = -33.2 kJ/mol) because of the ubiquity of inorganic pyrophosphatase (an enzyme that hydrolyzes PPi). Calculate the ΔG° for the overall reaction. c. What effect does the hydrolysis of PPi have on the formation of acetyl CoA?
Adi S.
The ΔG°' for the hydrolysis of acetyl-CoA to acetate and CoA is -32.2 kJ/mol, and that for the hydrolysis of ATP to AMP and PPi is -30.5 kJ/mol. Calculate ΔG°' for the ATP-dependent synthesis of acetyl-CoA.
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