Question

To prepare the protein assay reagent, the author dissolved Coomassie G-250 in 95% ethanol. Then, he added phosphoric acid. What was the purpose of adding the phosphoric acid? Hint: Pre-lab videos may help you answer this question. O Phosphoric acid will increase the pH and deprotonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is blue in color. Phosphoric acid will decrease the pH and protonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is red/brown in color. O Phosphoric acid will increase the pH and deprotonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is red/brown in color. Phosphoric acid will decrease the pH and protonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is blue in color. Phosphoric acid will decrease the pH and deprotonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is blue in color. O Phosphoric acid will increase the pH and protonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is blue in color. O Phosphoric acid will decrease the pH and deprotonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is red/brown in color. Phosphoric acid will increase the pH and protonate all of the sulfonate groups on the Coomassie G 250 dye. The protonated form of the dye is red/brown in color.

To prepare the protein assay reagent, the author dissolved Coomassie G-250 in 95% ethanol.
Then, he added phosphoric acid. What was the purpose of adding the phosphoric acid? Hint:
Pre-lab videos may help you answer this question.
O Phosphoric acid will increase the pH and deprotonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is blue in color.
Phosphoric acid will decrease the pH and protonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is red/brown in color.
O Phosphoric acid will increase the pH and deprotonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is red/brown in color.
Phosphoric acid will decrease the pH and protonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is blue in color.
Phosphoric acid will decrease the pH and deprotonate all of the sulfonate groups on
the Coomassie G 250 dye. The protonated form of the dye is blue in color.
O Phosphoric acid will increase the pH and protonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is blue in color.
O Phosphoric acid will decrease the pH and deprotonate all of the sulfonate groups on
the Coomassie G 250 dye. The protonated form of the dye is red/brown in color.
Phosphoric acid will increase the pH and protonate all of the sulfonate groups on the
Coomassie G 250 dye. The protonated form of the dye is red/brown in color.

Added by Olivia W.

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