Urease catalyzes the hydrolysis of urea into ammonia and CO2. The urease enzyme has a total molecular weight of 473 kDa.
The maximum rate of urea hydrolysis is 2.2 times faster at 25 °C than it is at 10 °C. What is ΔH?
b) The value of kcat is 560 s^-1 at 25 °C. Calculate ΔS.
c) In the absence of the enzyme, urea hydrolysis proceeds through an acid-catalyzed reaction mechanism with an activation energy of 105 kJ/mol. At what temperature would the acid-catalyzed urea hydrolysis proceed at the same rate as the 25 °C enzyme reaction? Assume ΔS is not affected by the mechanism.