Examine the images below of Vm data from current clamp...

Name: Examine the images below of Vm data from current clamp recordings. A sequential series of current injections ranging from 0 pA to -60 pA with a change of -15 pA each step is used to hyperpolarize these cells. Vm scale is the same for both cells. Which of these cells has higher resistance?
Uploaded: 2023-02-23T09:12:26-08:00
Duration: 6 min 20 s
Channel: Supreeta N
Description: Examine the images below of Vm data from current clamp recordings. A sequential series of current injections ranging from 0 pA to -60 pA with a change of -15 pA each step is used to hyperpolarize these cells. Vm scale is the same for both cells. Which of these cells has higher resistance?

Examine the images below of $V_m$ data from current clamp recordings. A sequential series of current injections ranging from 0 pA to -60 pA with a change of -15 pA each step is used to hyperpolarize these cells. $V_m$ scale is the same for both cells. Which of these cells has higher resistance?

Transcript

00:01 Hello students for the given question to demonstrate that a cloned gene produces or encodes a voltage gated sodium plus channel we would typically perform several voltage clamp experiments both in patch clamp and whole cell configurations patch clamp results in a patch clamp experiment a micropipet is used to create a small patch of membrane containing a single ion channel to test the voltage gating properties of the clone channel we would record the current flowing through the channel at various membrane potential first we would measure the current voltage relationship that is iv relationship of the channel by applying a series of voltage steps ranging from minus 100 to plus 100molt in 10 millivolt increments we would expect to see a steep increase in inward n -plus current as the membrane potential is depolarized, followed by a gradual decrease as the channel inactivates.

02:20 Next, we would access the kinetics of channel gating by applying a series of depolarizing voltage, steps and measuring.

02:41 The time course of current activation and the inactivation we would expect to see a fast rise time and relatively slow inactivation time consistent with the behavior of voltage gated sodium channels whole cell current results for whole cell current results recording the micropepet is used to break the cell membrane and excess the cytoplasm allowing the measurement of current flowing through all the ion channels in the cell to test whether the cloned channel is a voltage -gated any plus channel we would again measure the current voltage relationship by applying a series of voltage steps we would expect to see a large inward current at depolarized potentials similar to what we observed in the patch clamp experiments.

04:48 Next we would also access the kinetics of channel gating by applying a voltage step to depolarize the cell and then measuring the time course of current activation and inactivation...

Question

Examine the images below of $V_m$ data from current clamp recordings. A sequential series of current injections ranging from 0 pA to -60 pA with a change of -15 pA each step is used to hyperpolarize these cells. $V_m$ scale is the same for both cells. Which of these cells has higher resistance?

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