00:01
Let us consider pcr polymerase chain reaction which is also known as peeples -strauss reaction.
00:15
The instrument is thermocycler.
00:21
Polymerase chain reaction it is useful for amplification of desired dna.
00:32
Basically it works using three steps.
00:36
First step is denaturation.
00:41
Denaturation where double strand dna that is used for amplification is denatured, two strands are separated.
00:50
Second step is annealing.
00:52
The template dna is annealed with the primer and the third step is extension.
00:59
In extension the template dna is extended with the help of the dntps that are added into the buffer.
01:07
So as it is an exponential amplification of target dna, exponential amplification of target dna occurs using pcr because the cycle is repeated.
01:29
This repetition results in formation of multiple copies of target dna.
01:38
So initial step denaturation, first step where double strand dna is denatured at a temperature of 94 to 98 degree centigrade, two strands that are joined with the help of hydrogen bonds, they are separated at this high temperature.
02:05
This is first step that is denaturation of dna occurs.
02:11
In first step or initial step of dn, initial step of pcr...