when doing rflp analysis using pcr what result would you expect to see after gel electrophoresis

When doing rflp analysis using pcr what result would you...

Transcript

0:00 Hello students.

00:01 Now in this question they've given that there was rflp analysis that was done using the pcr.

00:07 After using the pcr they run on run the gel electrophoresis after which they observed that the result was quite different.

00:18 What was that happened in the result if they got to know that pcr did not did occur properly but the restriction digestion did not occur properly.

00:28 Now in this case what are we going to observe? let us consider three cases, okay? so here we will consider the three gels, right? what are the three gels? we will consider it as gel one, gel two and the gel three here, okay? in this case what we are going to be observing is that in the gel one, we are going to be considering all the right conditions which means pcr has occurred right and the rest of restriction endonuclease digestion has occurred properly.

01:02 So here if we observe let us consider three wells, okay? and i have repeated the experiment twice and both the times the answer has come.

01:12 So the first one, this here i will mark one, two, three wells.

01:16 So the first one we are observing that the first one will be the dna ladder.

01:21 Okay.

01:22 The restriction endonuclease that we are going to be observing in the second and the third case, we will observe it somewhat like this.

01:29 Okay.

01:30 Let us say that there are three distinct bands that we observe because there are only three restriction sites in this particular band, okay? or particular sample.

01:40 So here we observe this is the right case.

01:43 Okay...

Question

When doing RFLP analysis using PCR, what result would you expect to see after gel electrophoresis if the PCR was successful but the restriction digest fails?

Question

When doing RFLP analysis using PCR, what result would you expect to see after gel electrophoresis if the PCR was successful but the restriction digest fails?

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