When performing a liquid injection onto a GC system what considerations regarding the sample must be made to ensure efficient chromatography?
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1. Under what experimental conditions is it necessary to use liquid chromatography instead of gas chromatography? Provide 3 reasons to justify answer. 2. If a polar stationary phase is used and you inject a sample which contains butanol and hexane, which compound would elute first and why? What would happen if we used a reverse phase column? 3. Suppose you are preparing to run a sample in the GC and you first rinse the syringe with methanol. What would you expect to see on the chromatogram if you did not properly expel all of the methanol and it became mixed with your sample?
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