'Why did we have to re-calibrate the spectrometer back to zero at each different wavelength if all we had in the blank cuvette was water?'
Added by Olga F.
Step 1
Step 1: Distilled water, even though it appears pure, can still contain impurities such as ions that can absorb light at certain wavelengths. Show more…
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Adi S.
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Why is it necessary to run a blank before measuring the absorbance of a sample? Select one: So that scattered light will be filtered before hitting the photodetector: To account for the absorbance caused by the cuvette and the solvent To allow the lamp and electronics of the spectrophotometer to warm up before taking the sample absorbance reading: None of the answers is correct To account for the absorbance caused by the cuvette alone
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