Why Sanger sequencing uses ddNTPs and how they differ functionally from dNTPs. Does Sanger sequencing minimize the length of replication since phosphodiester bonds are not forned during DNA replication. Also, does it differ from dNTPs functionally since it lacks two hydroxyl groups?
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These ddNTPs differ functionally from dNTPs (deoxynucleotide triphosphates) because they lack the 3'-hydroxyl group on the sugar moiety, which is essential for the formation of phosphodiester bonds between nucleotides during DNA replication. In Sanger sequencing, Show more…
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