7. You are monitoring a reaction using Thin Layer Chromatography (TLC). Each TLC plate contains a spot for the starting materials (SM), the reaction (RXN), and the expected product (P). RXN samples were collected at t = 0, 5, 15, and 25 min, as indicated at the top of each TLC plate. a) Evaluate the developed TLC plates and determine the ideal time (0, 5, 15, or 25 min) to stop this reaction. (1 pts) b) Justify your choice in one sentence. (2 pts) t = 0 min t = 5 min t = 15 min t = 25 min SM RXN P SM RXN P SM RXN P SM RXN P
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We are looking for the point at which the starting materials (SM) have mostly disappeared and the expected product has appeared. Show more…
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Thin layer chromatography (TLC) is used all the time to monitor reactions. Below is a typical TLC plate when viewed under UV light, showing a reaction that is almost complete. Note that when we monitor a reaction, we always include a 'mixed spot' (mix) that has both the starting material (SM) and the reaction products (react) in it. Sometimes the starting material and product can have very similar Rf values, so a mixed spot can help us 'spot' this. For the TLC plate below, fill in the Rf values (to two decimal places) for the starting material (SM) and the product (new spot seen in the 'react' spot).
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