Question

You have run the RP-HPLC with your samples and have obtained the following chromatogram: 140 120 105 90 75 60 45 30 15 0 7.5 15.0 22.5 30.0 37.5 45.0 52.5 56.0 K-CN \alpha_{s1}-CN \alpha_{s1}-CN \beta-CN \beta-LgB \beta-LgA \alpha-La min 3. (0.5 marks) According to the chromatogram, which is the most hydrophobic protein and why? 4. (0.5 marks) Which alleles (genotype) do you think the cow, whose milk has been analysed in the chromatogram, has for beta-lactoglobulin?

          You have run the RP-HPLC with your samples and have obtained the following chromatogram:
140
120
105
90
75
60
45
30
15
0
7.5
15.0
22.5
30.0
37.5
45.0
52.5
56.0
K-CN
\alpha_{s1}-CN
\alpha_{s1}-CN
\beta-CN
\beta-LgB
\beta-LgA
\alpha-La
min
3. (0.5 marks) According to the chromatogram, which is the most hydrophobic protein and
why?
4. (0.5 marks) Which alleles (genotype) do you think the cow, whose milk has been
analysed in the chromatogram, has for beta-lactoglobulin?

You have run the RP-HPLC with your samples and have obtained the following chromatogram:
140
120
105
90
75
60
45
30
15
0
7.5
15.0
22.5
30.0
37.5
45.0
52.5
56.0
K-CN
αs1-CN
αs1-CN
β-CN
β-LgB
β-LgA
α-La
min
3. (0.5 marks) According to the chromatogram, which is the most hydrophobic protein and
why?
4. (0.5 marks) Which alleles (genotype) do you think the cow, whose milk has been
analysed in the chromatogram, has for beta-lactoglobulin?

Added by Sylvia R.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Riaz Ahmad

Step 1

In RP-HPLC, the more hydrophobic a protein is, the longer it will take to elute from the column. Looking at the retention times in the chromatogram, we can see that the protein with the longest retention time is 8-LgB, which elutes at 52.2 minutes. Therefore, Show more…

Show all steps

Thanks for your feedback!

You have run the RP-HPLC with your samples and have obtained the following chromatogram: 8-LgB B-LA 22.5 37.5 45.0 522 3.0.5 marks According to the chromatogram, which is the most hydrophobic protein and why? 4.0.5 marks Which alleles/genotype do you think the cow whose milk has been analyzed in the chromatogram has for beta-lactoglobulin?