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Understanding Biology

Kenneth A. Mason, Tod Duncan, George B. Johnson

Chapter 17

Biotechnology - all with Video Answers

Educators


Section 1

Enzymes Allow the Creation of Recombinant Molecules In Vitro

01:41

Problem 1

The palindromic recognition sequence for the restriction enzyme BamH1 is $5^{\prime}$ GGATCC $3^{\prime} .$ Remember, the other strand of DNA will have the complementary set of bases and will read the same as this sequence, but just "backwards." The enzyme cuts between the first and second Gs in the sequence. What is the "sticky-end" sequence when the enzyme cuts double-stranded DNA?
a. $5^{\prime}$ GGATTC $3^{\prime}$
c. $5^{\prime}$ GATCC $3^{\prime}$
b. $5^{\prime}$ CCATGG $3^{\prime}$
d. $5^{\prime}$ CCATG $3^{\prime}$

Joanna Quigley
Joanna Quigley
Numerade Educator
03:42

Problem 2

What is the basis of separation of different DNA fragments by gel electrophoresis?
a. The positive charge on DNA
b. The size of the DNA fragments
c. The sequence of the fragments
d. The presence of a dye in the gel

Eric Tran
Eric Tran
Numerade Educator