Nester's Microbiology: A Human Perspective

Denise Anderson, Sarah Salm, Deborah Allen

Chapter 9

Biotechnology and Recombinant DNA - all with Video Answers

Educators

Chapter Questions

Problem 1

What is the function of a vector?
a) Destroys cells that do not contain cloned DNA
b) Allows cells to take up foreign DNA
c) Carries cloned DNA, allowing it to replicate in cells
d) Encodes herbicide resistance
e) Encodes Bt toxin

Sylvia Puglisi

Sylvia Puglisi

Numerade Educator

Problem 2

The Ti plasmid of Agrobacterium tumefaciens is used to genetically engineer which of the following cell types?
a) Animals b) Bacteria
c) Plants
d) Yeast
e) All of these

Sylvia Puglisi

Numerade Educator

Problem 3

Which of the following can be used to generate a DNA library?
a) $\mathrm{PCR}$
b) Sequencing
c) Colony blotting
d) Microarrays
e) Cloning

Sylvia Puglisi

Numerade Educator

Problem 4

An ideal vector has all of the following except
a) an origin of replication.
b) a gene encoding a restriction enzyme.
c) a gene encoding resistance to an antibiotic.
d) a multiple-cloning site.
e) the $\operatorname{lac} Z$ ' gene.

Sylvia Puglisi

Numerade Educator

Problem 5

Which of the following describes the function of the $\operatorname{lac} Z^{\prime}$ gene in a cloning vector?
a) Means of selecting for cells that contain vector sequences
b) Means of distinguishing cells that have taken up recombinant molecules
c) Site required for the vector to replicate
d) Mechanism by which cells take up the DNA
e) Gene for a critical nutrient required by transformed cells

Sylvia Puglisi

Numerade Educator

Problem 6

Which is used for cloning eukaryotic genes but not prokaryotic genes?
a) Restriction enzymes
b) DNA ligase
c) Reverse transcriptase
d) Vector
e) Selectable marker

Sylvia Puglisi

Numerade Educator

Problem 7

Which of the following does a dideoxynucleotide lack?
a) $5^{\prime} \mathrm{PO}_{4}$
b) $3^{\prime} \mathrm{OH}$
c) $5^{\prime} \mathrm{OH}$
d) $3^{\prime} \mathrm{PO}_{4}$
e) $c$ and $d$

Sylvia Puglisi

Numerade Educator

Problem 8

In a sequencing reaction, the dATP was left out of the tube. What would be the result of this error?
a) No synthesis would occur.
b) Synthesis would never continue past the first A.
c) Synthesis would not stop until the end of the template.
d) Synthesis would terminate randomly, regardless of the nucleotide incorporated.
e) The error would have no effect.

Sylvia Puglisi

Numerade Educator

Problem 9

The polymerase chain reaction uses Taq polymerase rather than a DNA polymerase from $E$. coli, because Taq polymerase
a) introduces fewer errors during DNA synthesis.
b) is heat-stable.
c) can initiate DNA synthesis at a wider variety of sequences.
d) can denature a double-stranded DNA template.
e) is easier to obtain.

Sylvia Puglisi

Numerade Educator

Problem 10

The polymerase chain reaction generates a fragment of a distinct size even when an intact chromosome is used as a template. What determines the boundaries of the amplified fragment?
a) The concentration of one particular deoxynucleotide in the reaction
b) The duration of the elongation step in each cycle
c) The position of a termination sequence, which causes the $T a q$ polymerase to fall off the template
d) The sites to which the primers anneal
e) The temperature of the elongation step in each cycle

Sylvia Puglisi

Numerade Educator