Chapter Questions
Which of the following facilitates joining a piece of human DNA with bacterial plasmid DNA?a. DNA polymerasec. RNA polymeraseb. DNA ligased. restriction enzymes
Which of the following acts like a molecular copying machine, rapidly making large numbers of specific sequences of DNA?a. DNA fingerprintingc. DNA sequencingb. DNA ligased. polymerase chain reaction
Which statement regarding plasmids is false?a. They can be easily extracted from bacteria.b. They can be reinserted into bacterial and plant cells.c. They can replicate inside the recipient cell.d. They can be easily introduced into animal cells.
Genetically engineered human insulin, human growth hormone, and human clotting factor VIII are made by:a. gel electrophoresisb. polymerase chain reactionc. transgenic bacteriad. DNA fingerprinting
Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes.
All of the following statements about gene therapy are true except:a. It can successfully treat SCID.b. Retroviruses insert their genetic material randomly.c. Its purpose is to modify embryos according to the parents" wishes.d. It may soon be able to treat cancer.
"Gene pharming" refers to:a. using transgenic farm animals to produce human proteins for medical usesb. producing transgenic plants with improved nutritional valuec. producing drugs using transgenic plantsd. using transgenic plants to produce vaccines
Which of the following poses the greatest challenge to gene therapy?a. identifying the chromosomal location of the defective geneb. obtaining a normal allele of the defective genec. producing multiple copies of the normal allele to replace the defective alleled. delivering the normal allele into the cells of the recipient
What would be the advantage of introducing genes into germ cells rather than somatic cells?a. Genes can be more readily inserted into germ cells.b. Introduced genes are more often lost from somatic cells.c. Genes introduced into germ cells are more readily expressed.d. Genes introduced into germ cells would be passed on to gametes and future generations.
The role of restriction enzymes in bacterial cells is to:a. assist in DNA replicationb. process primary transcripts into mRNAc. destroy invading viruses by cutting up their DNAd. join pieces of DNA
Which of the following is needed for sequencing DNA?a. DNA polymerasec. primersb. gel electrophoresisd. all of these choices
A tiny amount of blood was recovered from a crime scene. What technique will enable comparison of this DNA to that of a potential suspect?a. DNA fingerprintingc. DNA sequencingb. DNA cloningd. gene therapy
Which technique will allow amplification of the DNA from the crime scene, in order to provide a large enough specimen?a. gel electrophoresisc. transgenic introductionb. polymerase chain reactiond. DNA sequencing
A gene for a bacterial protein with insecticide properties has been introduced into a plant. This plant is now referred to as a(n):a. insectivorous plantc. genetically modified plantb. transgenic plantd. both (b) and (c)
Foreign DNA is introduced into an animal cell by:a. microinjecting DNAc. both (a) and (d)b. plasmidsd. silicon needles