Painting: Charles Vwas very rich but very ill. He looks like he has a glove and that's because he wants to hide some
deformities that he held in his hand. I wanted to cover up the fact that I have arthritis. It was not known if he had gout.
At the end of his days he had great difficulty walking and doing everything. There came a time when he couldn't even sign.
It was determined that he did in fact have a drip, but he did not die from it. Clinical hospital investigators requested a sample of his mummified body. They took a sample of the finger and saw that there were some needle-shaped crystals that provide the differential diagnosis to confirm that a person has gout.
You published a very important medical paper in 2006.
Purines are nitrogenous bases that are part of nucleic acids. The purine bases would be adenine and guanine.
The nucleotide is the nucleoside with a phosphate group.
Of purines we could talk about hypoxanthine (at first it was said to be just a metabolite, and now DNAs have been found with itfi.
There are derivatives of nucleotides like ATP and ADP or of signal transducing molecules like GTP.
uric acid metabolism
End product of purine metabolism.
There are three sources of manure:
Synthesis again. It is synthesized from a molecule of different types. tissue rupture meal
The synthesis is based on ribose - 5P, which is derived from glucose (pentose phosphate pathwayfi. There's an enzyme that incorporates two more phosphates, and we get PRPP. This one has 5P and the other two have phosphates (pyrophosphatefi Another reaction adds another amine and then a whole series of reactions occur.
Ribosa-5P PRPP-sintetasa PRPP PRPP-amidotransferasa
Sintesis
AMP 5'N! ISN ADA Adenosina inosina PNP Degradacion Hipoxantina 1x0
GMP ISN Guanosina PNP Guanina Guanasa
It comes to IMP and then to AMP and GMP. If the cell has oversynthesized or just created them and they continue to be degraded, degradation would begin with nucleotidases
Adenosine can be converted to inosine by ADA, while guanosine follows its own breakdown.
1x0 Acido urico
Inosine will continue its degradation thanks to PNP and will end Figura 18-2.Resumen del metabolismo de los nucleotidos purinicos.ADA adenosina-desaminasa; AMP: adenosina monofosfato;GMP:guanosina mo- up in hypoxanthine. And from the XO (xanthine oxidasefi, which nofosfatoIMP:inosina monofosfato;5'N:5-nucleotidasaPNP:purina-nu cleosido-fosforilasa; PRPP:5-fosforribosil-pirofosfato;XO:xantina-oxidasa ends up giving xanthine and then uric acid. Gonzalez Hermandez,A.Principios de BQClin.Elsevier 2010
With these synthetic pathways, there are some escape routes after degradation. These exist in many biochemical pathways, but this is where it will be very interesting.
The cell can create metabolites and break them down or reuse them. All of this will be very dynamic and needs will change rapidly.
Once breakdown begins and we have the purine bases, many cells reuse them to make nucleic acids. They do this thanks to various enzymes such as HGFRT.This enzyme takes the base and adds 5P-ribose to it. The cell will do this when it needs to make nuclei