PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability
Activity 5: Simulating Active Transport Name: Sara Ross Date: 27 January 2023
Session ID: session-4eac699b-ec69-aa18-62dc-97f4adc663e8
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1 The movement of sodium and potassium maintained by the Na *-K *pump
You correctly answered: requires energy and is against a concentration gradient.
2 The sodium-potassium pump is classified as a(n)
You correctly answered: antiporter.
3 The sodium-potassium pump moves simultaneously.
sodium ions and
potassium ions
You correctly answered: 3, 2.
You correctly answered: lipid insoluble
Experiment Results
Predict Questions
1 Predict Question 1: What do you think will result from these experimental conditions?
Your answer: Na* will be maximally transported
2 Predict Question 2: Do you think the addition of glucose carriers will affect the transport of
sodium or potassium?
Your answer: No, it will not affect the transport of either ion.
Stop & Think Questions
1 Why did the sodium transport stop before the transport was completed?
You correctly answered: The ATP was depleted.
2 WhycarethryeauieriumfRethweolutesrepcheosrrlieransport
Experiment Data
Run Number
Solute
ATP (mM) Start Conc. L (mM) 1 9.000
Start Conc. R (mM) 0.000
Pumps
Carriers
Rate (mM/sec)
1 1
Na* CI- K* CI- Glucose Na* CI- K+ CI- Glucose Na* CI- K* CI- Glucose Na* CI- K*CI- Glucose Na* CI- K* CI- Glucose
500
---
0.0188
1
0.000 0.000 9.000
6.000 0.000 0.000 6.000 0.000
500
---
0.0125 0.0000 0.0025
---
0
--
2 2 2 3 3 3 4
3 3
500
---
0.000
500
---
0.0017
-- 3 3
0.000
---
0
0.0000
9.000 0.000 0.000
0.000 0.000 0.000
500 500
---
0.0000 0.0000 0.0000
---
--- 3 3
-
0
9.000
0.000
800 800
---
0.0042
4 4
0.000 0.000
6.000 0.000
---
0.0028 0.0000
--- 3 3
--
0
5 5 5
9.000
0.000
800 800
---
0.0042
0.000
6.000
--
0.0028
---
0.000
0.000
---
400
0.0000
Post-Iab Quiz ResuIts
You scored 100% by answering 4 out of 4 questions correctly
1 What happened when you increased the amount of ATP dispensed with the same
You correctly answered: More ions were transported.
2 At what concentration of ATP were the sodium and potassium maximally transported?
You correctly answered: 3 mM ATP
3 What was the effect of adding more Na*-K*pumps to the simulated cell?
4 Describe the effect of adding glucose carriers to the sodium and potassium transport.
You correctly answered: There was no change in the transport rate because glucose is transported independently.
Review Sheet Results
1 Describe the significance of using 9 mM sodium chloride inside the cell and 6 mM potassium chloride outside the cell, instead of other concentration ratios.
Your answer:
we knew the ratio for the pump was 3:2, so doing some math and matching the ratios the 9mM:6mM ratio is the most beneficial
2 Explain why there was no sodium transport even though ATP was present. How well did the results compare with your prediction?
You