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PhysioEx Lab Report in Introductory Biology

PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 2: Simulated Facilitated Diffusion Name: Sara Ross Date: 27 January 2023 Session ID: session-0f7b2b9f-cd61-a567-2e3a-3363926e7184 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Molecules need a carrier protein to help them move across a membrane because You correctly answered: they are lipid insoluble or they are too large. 2 Which of the following is true of facilitated diffusion? You correctly answered: Movement is passive and down a concentration gradient 3 Examples of solutes that might require facilitated diffusion include You correctly answered: all of the above 4 Which of the following would not affect the rate of facilitated diffusion? You correctly answered: the amount of intracellular ATP Experiment Results Predict Questions 1 Predict Question 1: What effect do you think increasing the number of protein carriers wil have on the glucose transport rate? Your answer: The glucose transport rate will decrease. 2 Predict Question 2: What effect do you think adding Na 'Cl -will have on the glucose transport rate? Your answer: The glucose transport rate will decrease Stop & Think Questions 1 What happened to the glucose transport rate when the glucose concentration was increased from 2 mM to 8 mM? Your answer: The glucose transport rate decreased Correct answer: The glucose transport rate increased 2 Describe the conditions that resulted in the fastest rate of glucose transport You correctly answered: 700 glucose carrier proteins and 8 mM glucose 3 Why do you think equilibrium wasn't reached? You correctly answered: The membrane proteins were saturated Experiment Data Run Number Solute Start Conc. L Start Conc. R (mM) (mM) 0.00 0.00 Carriers Rate (mM/sec) 0.0000 1 1 Na* CI- 500 500 500 500 700 700 700 700 Glucose Na* CI- Glucose Na* CI- Glucose Na* CI- Glucose Na* CI- Glucose Na* CI- Glucose 2.00 0.00 8.00 0.00 0.00 0.00 0.00 0.00 0.0008 2 2 3 3 4 4 5 5 6 6 0.0000 0.0023 0.0000 2.00 0.00 0.0010 0.00 8.00 0.00 0.00 0.0000 0.0031 0.00 10.00 0.00 0.00 100 100 0.0000 0.0017 0.00 2.00 2.00 0.00 700 700 0.0042 0.0010 Post-Iab Quiz Results You scored 100% by answering 4 out of 4 questions correctly 1 What variable(s) increased the rate of facilitated diffusion of glucose? You correctly answered: increasing the concentration of glucose and increasing the number of membrane carriers. 2 For facilitated diffusion, increasing the concentration of glucose on one side of the membrane is the same as You correctly answered: making the concentration gradient steeper. 3 When all of the membrane carriers are engaged, or busy, we say they are You correctly answered: saturated. 4 Na*cI- had no effect on glucose transport because You correctly answered: sodium is not required for glucose transport in the simulation. Review Sheet ResuIts 1 Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it is different from simple diffusion. Your answer: They are the same