PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability
Activity 1: Simulating Dialysis (Simple Diffusion) Name: Sara Ross Date: 27 January 2023
Session ID: session-9bbe5210-6461-a42d-18e9-67a71fc8be8a
Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
1 The driving force for diffusion is
You correctly answered: the kinetic energy of the molecules in motion.
2 In diffusion, molecules move
You correctly answered: from high concentration to low concentration
3 Which of the following dialysis membranes has the largest pore size?
You correctly answered: 200 MWCO
Experiment Results
Predict Questions
1 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer: Yes, but very slowly.
2 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607
amino acids, and the average molecular weight of a single amino acid is 135 g/mol
Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer: glucose only.
Stop & Think Questions
1 The reason sodium chloride didn't diffuse left to right is that
You correctly answered: the membrane pore size was too small.
2 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average
molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes
at the 20 MWCO because
Your answer: albumin is a simple sugar and therefore too large to pass
Correct answer: glucose and albumin are both too large to pass
3 The rate of diffusion for urea
You correctly answered: is slower than that for sodium because urea is a larger molecule
Experiment Data
Solute
MWCO
Left Solute (mM)
Right Solute (mM)
Average Diffusion (mM/sec) 0.0000
Na* CI-
20 20 20 20 20 20 20 20 50 50 50 50 50 50 50 50 100 100 100 100 100 100 100
9.00
0.00
Urea Albumin Glucose
0.00
0.00
0.0000
0.00 0.00 0.00 9.00 0.00
0.00 0.00 0.00
0.0000 0.0000
Na* CI-
0.0000
Urea Albumin Glucose Na* CI- Urea Albumin Glucose Na* CI-
0.00 0.00
0.0000 0.0000
0.00
0.00
0.0000
9.00
0.00
0.0150
0.00
0.00
0.0000
0.00 0.00 18.00
0.00 0.00 0.00
0.0000
0.0000 0.0273
Urea Albumin Glucose Na* CI- Urea Albumin Glucose Na* CI- Urea Albumin
0.00
0.00
0.0000
0.00
0.00
0.0000
0.00 9.00 0.00
0.00 0.00 0.00
0.0000 0.0150
0.0000
0.00 0.00
0.00 0.00
0.0000
0.0000
0.00 9.00 0.00
0.00 0.00 0.00
0.0000 0.0094
0.0000
Glucose Na* CI- Urea Albumin Glucose Na* CI-
100 200 200 200 200 200
0.00 0.00 0.00 0.00 9.00 0.00 0.00 9.00 0.00
0.00 0.00 0.00 0.00
0.0000 0.0000
0.0000
0.0000
0.00 0.00
0.0042
0.0000
Urea Albumin Glucose
200 200 200
0.00 0.00 0.00
0.0000
0.0000 0.0000
Post-Iab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly
1 The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
You correctly