BIOMARKERS Biomarkers Taylor & Fr Taylor & Francis Grou ISSN: (Print) (Online) Journal homepage: www.tandfonline.com/journals/ibmk20 Neuroprotective effects of magnesium against stress induced by hydrogen peroxide in Wistar rat Latifa Hajri, Haifa Othman, Soumaya Ghodbane, Mohsen Sakly, Hafedh Abdelmelek, Khemais Ben Rhouma & Mohamed Ammari To cite this article: Latifa Hajri, Haifa Othman, Soumaya Ghodbane, Mohsen Sakly, Hafedh Abdelmelek, Khemais Ben Rhouma & Mohamed Ammari (2023) Neuroprotective effects of magnesium against stress induced by hydrogen peroxide in Wistar rat, Biomarkers, 28:6, 538-543, DOI: 10.1080/1354750X.2023.2246104 To link to this article: https://doi.org/10.1080/1354750X.2023.2246104 Published online: 27 Aug 2023. Submit your article to this journal C Article views: 103 O View related articles CrossMark View Crossmark data C Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=ibmk20
BIOMARKERS 2023, VOL. 28, NO. 6, 538-543 https://dOI.ORg/10.1080/1354750X.2023.2246104 ORIGINAL ARTICLE Taylor & Francis Taylor & Francis Group Check for updates Neuroprotective effects of magnesium against stress induced by hydrogen peroxide in Wistar rat Latifa Hajria, Haifa Othmanª, Soumaya Ghodbanea, Mohsen Saklya (D, Hafedh Abdelmelekª, Khemais Ben Rhoumaª and Mohamed Ammaria,b# AFAculty Of SclEncES Of BIzERtE, LABORAtORy Of IntEgRAtIvE PhySIOIOgy, UnlvERSIty Of CARthAgE, JARzOunA, TunISIA; bHighER InStitutE Of AppllEd BIOIOgIcAl SclEncES Of TunIS, UnlvERSIty Of TunIS EI MANAR, TunIS, TunISIA ABSTRACT Introduction: Oxidative stress has been implicated in the pathogenesis of diverse disease states. The present study was designed to examine the effects of magnesium sulphate (MgSO4) against hydrogen peroxide (H2O2) induced behaviour impairment and oxidative damage in rats. Material and methods: Eighteen rats were equally divided into three groups. The first group was kept as a control. In the second group, H2O2 was given in drinking water at 3% during 5 days. In the third group, rats were subjected to daily administration of H2O2 and MgSO4 (100mg/kg; b.w) for 5 days. Animals were subjected to behavioural tests (elevated plus maze and open field). At the end of experiment, brains were extracted for oxidative stress biomarkers assessment including levels of malondialdehyde and hydrogen peroxide and activities of superoxide dismutase and catalase. Results: Our findings showed that H2O2 treated rat exhibited anxiogenic behaviour and the genesis of free radicals in the brain. Magnesium showed amelioration against oxidative stress and significant decrease in anxiety levels. Discussion and conclusion: Stress is a powerful process that disrupts brain homeostasis by inducing oxidative stress and its appear that magnesium may have potential therapeutic benefits by reducing oxidative stress and inducing anxiolytic effect. ARTICLE HISTORY Received 23 May 2023 Accepted 4 August 2023 KEYWORDS Biomarkers; magnesium sulphate; oxidative stress; anxiety; rat 1. Introduction Stress is known as any circumstance potentially able to dis- rupt the physiological or psychological homeostasis (Kour and Bani 2011). A hallmark of the stress response is the acti- vation of the hypothalamus-pituitary-adrenal (HPA) axis. The HPA Axis is affected by both internal physiological stressors (cytokines, hypoxia, macromolecules etc.) and by external stressors (source of anxiety, fear etc.) which threaten the organism's homeostasis (Patricia Gaete 2016) Although this physiological phenomenon is fundamental to survival, it is also strongly