Professor Brian Key . School of Biomedical Sciences Developmental Biology · brian.key@uq.edu.au For additional reading (above and beyond what has been placed on Blackboard) please refer to Gilbert's Developmental Biology 9th Edition (or equivalent in other books). Please note that since the text covers more than the lectures. Only the lecture material is required knowledge. The reading should enable you to better understand the concepts presented in class. This reading is meant to provide you with a solid understanding of some of the basic principles that underlie Developmental Biology. Introducing/Questions of Developmental Biology pp 1-4 Developmental Anatomy pp 5-6 Naming the parts p 14 Fate maps pp 17-19 Morphogenesis and cell adhesion pp 69-72 Cell signalling pp 79-80 Conditional specification pp 112-113 Morphogen gradients p 116-117 Stem cells pp 323-325 1
What is development? What is it good for? O Julius T. Csolonyi Somatic cell nuclear transfer (cloning) - a source of cloned cells for therapy Fibroblast 1 Blastocyst SCNT NT-ESCs Oocyte Differentiation Pancreatic islet cells Hematopoietic cells Cardiomyocytes Hepatocytes Neurons In 1996, Roslin Institute and collaborators in PPL Therapeutics created Dolly, the first animal cloned from a cell (udder) taken from an adult animal. Success rates remain low in all species since cloned (mice , rabbits, horses, donkeys, pigs, goats and cows) with published data showing that on average only about 1% of 'reconstructed embryos' leading to live births. 2
SCNT - animals for disease models & therapy Cell 172, 881-887, February 8, 2018 @ 2018 Elsevier Inc. 881 Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer Zhen Liu,1 Yijun Cai,' Yan Wang,1 Yanhong Nie,' Chenchen Zhang,1 Yuting Xu,1 Xiaotong Zhang,1 Yong Lu,1 Zhanyang Wang,1 Muming Poo,1 and Qiang Sun1,2,+ Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, State Key Laboratory of Neuroscience, CAS Key Laboratory of Primate Neurobiology, Chinese Academy of Sciences, Shanghai, China ELSEVIER Original article Cloning of animals by somatic cell nuclear transfer (SCNT) has been achieved in 23 mammalian species (see review Rodri- guez-Osorio et al., 2012), including sheep (Wilmut et al., 2007), mouse (Wakayama et al., 1998), cattle (Kato et al., 1998), pig (Polejaeva et al., 2000), cat (Shin et al., 2002), rat (Zhou et al., 2003), and dog (Lee et al., 2005). As species closer to humans, non-human primates are ideal animal models for studying physiological functions unique to primates and for developing therapeutic treatments of human diseases (Izpisua Belmonte et al., 2015; Jennings et al., 2016). Animal models with genetic uniformity are often desirable (Schramm and Paprocki, 2004), Reproductive Biology 18 (2018) 18-26 Contents lists available at ScienceDirect REPRODUCTIVE Reproductive Biology journal homepage: www.elsevier.com/locate/repbio - Scriptaid improves the reprogramming of donor cells and enhances canine- porcine interspecies embryo development Jin-Gu Noa,b,1, Tai-Young Hura,1, Minghui Zhaoª, Seunghoon Leeª, Mi-Kyung Choi“, Yoon-Seok Nama, Dong-Hyun Yeom", Gi-Sun Im2, Dong-Hoon Kima .* " Department of Animal Biotechnology, Nazona! InszÃnze of Animal Science, Warju 55365, Republic of Korea Department of Biological Science, University of Sungkyunkwan, Suwon 16419, Republic of Korea Table