EDVOTEK ® AP BIOLOGY BIG IDEA 3 INVESTIGATION 7 EDVO-Kit: AP07 Cell Division: Mitosis and Meiosis See Page 3 for storage instructions. EXPERIMENT OBJECTIVE: The objective of this experiment is for students to identify and differentiate various stages in mitosis and meiosis. Onion root tips are stained to identify the various stages and duration of mitosis. Students will also have an opportunity to analyze mechanism involved with loss of cell cycle control in cancer. Meiosis and Crossing Over in Sordaria is also examined in this experiment. 1 . 800 . EDVOTEK www.edvotek.com info@edvotek.com EVT AP07.121213
EXPERIMENT AP07 Cell Division: Mitosis and Meiosis Table of Contents Page Experiment Components 3 Experiment Requirements 3 Background Information 4 Experiment Procedures Experiment Overview 12 Investigations I & IV: Modeling Mitosis and Meiosis 13 Investigation II: Studying the Effects of Environment on Mitosis 17 Investigation III: Loss of Cell Cycle Control in Cancer 19 Investigation V: Meiosis and Crossing Over in Sordaria 22 Study Questions 23 Instructor's Guidelines 25 Notes to the Instructor Pre-Lab Preparations 26 Experiment Results and Analysis 28 Study Questions and Answers 32 Material Safety Data Sheets 34 The Advanced Placement (AP) Program is a registered trademark of the College Entrance Examination Board. These laboratory materials have been prepared by EDVOTEK, Inc. which bears sole responsibility for their contents. All components are intended for educational research only. They are not to be used for diagnostic or drug pur- poses, nor administered to or consumed by humans or animals. THIS EXPERIMENT DOES NOT CONTAIN HUMAN DNA. None of the experiment components are derived from human sources. EDVOTEK and The Biotechnology Education Company are registered trademarks of EDVOTEK, Inc. EDVOTEK® The Biotechnology Education Company® . 1-800-EDVOTEK . www.edvotek.com 2 Duplication of any part of this document is permitted for non-profit educational purposes only. Copyright @ 1989-2012 EDVOTEK, Inc., all rights reserved. EVT AP07.121213
Cell Division: Mitosis and Meiosis EXPERIMENT AP07 Experiment Components Investigations I & IV · 3-cm long Pipe cleaners (in 2 colors) . . Small plastic bags Beads Investigations II · Carbol-fuschin (Ziehl-Neelson) stain · Lectin (phytohemagglutinin PHA-M from Phaseolus vulgaris) · Microscope slides and covers • 10 plastic cups (to grow onion root tips) · Sand · 50 ml conical tubes Investigations III · Karyotype pictures of normal individuals · Karyotype pictures of patients 1, 2, and 3 Investigations V · Pictures of Sordaria fimicola (Color pictures recommended) Requirements Investigations I & IV · Colored pencils (2 colors) Store the entire experiment at room temperature. This experiment is designed for 10 lab groups. Investigation II • Microscope • 10 green onions (or scallions) with roots • Ethanol · Glacial acetic acid (12 M) · Hydrochloric acid · Razor blades • Scissors • Scientific cleaning wipes (Kimwipes) · Disposable gloves Investigations III & V • Photocopier The Biotechnology Education Company® . 1-800-EDVOTEK . www.edvotek.com EDVOTEK® 3 Duplication of any part of this document is permitted for non-profit educational purposes only. Copyright @ 1989-2012 EDVOTEK, Inc., all rights reserved. EVT AP07.121213