Sets of MCQs to discuss Session 10: Introduction to molecular biology Question 1: Which of the following chromosomes is metacentric a) Chromosme 1 b) Chromosme 7 c) Chromosme 10 d) Chromosme 14 e) Chromosme 3 Karyotype 1 2 3 4 5 2 3 . 6 7 8 9 10 11 12 13 14 15 16 17 10 18 = 12 13 14 15 16 17 18 19 20 21 22 19 20 21 22 Humans contain 23 pairs of linear chromosomes 6 x 109 bases in the diploid state Question 2: What are you not expected to find in a plasmid used for eukaryotic gene expression a) A bacterial origin of replication b) A promoter for bacterial expression of specific proteins c) A multicloning site with bacterial restriction enzymes d) A bacterial gene involved in specific metabolism e) A bacterial operon Question 3: Which one of the following best describes the pairing which will result in a successful ligation experiment? a) An unphosphorylated piece of plasmid DNA and phosphorylated insert, both with complementary end base pairs b) A phosphorylated piece of plasmid DNA and phosphorylated insert, both with complementary ends c) A phosphorylated piece of plasmid DNA and phosphorylated insert, both with blunt ends d) An unphosphorylated piece of plasmid DNA and phosphorylated insert, both with blunt end base pairs e) All of the above Session 11: Translation Question 2: Which of the following translation steps is the most rate limiting one 1. Initiation 2. Elongation 3. Termination 4. mRNA maturation 5. All of the above Question 3: Which of the following translation steps contains the most factors in prokaryotes 1. Initiation 2. Elongation 3. Termination 4. Ribosome 5. All of the above Question 4: Which of the following translation steps contains the most factors in eukaryotes 1. Initiation 2. Elongation 3. Termination 4.Ribosome 5.All of the above Question 5: Do you find Shine Dalgarno sequences in eukaryotic mRNAs? 1. Yes 2. No
Questions to discuss as groups: 2. Which ONE of the 3 steps in protein translation is the most conserved across both prokaryotic and eukaryotic organisms? Session 12: Protein structure Kahoot quiz Session 13: Cytoskeleton Question 2: which one of the following does not have a cytoskeletal component 1. Erythrocyte 2. Neuron 3. Monocyte 4. Cov- SARS II 5. E. coli Question 3: Which statement is incorrect? 1. Any living cell, whether prokaryote or eukaryote, has a cytoskeleton 2. There are only 3 types of cytoskeletal proteins 3. Nuclei contain cytoskeletal proteins 4. A cytoskeletal fibre can be much longer than a cell 5. Organelles can use the cytoskeleton to move in cells Question 4: Which one of the following statements is INCORRECT 1) Microfilaments are made of actin 2) Actin polymerisation is induced by 100mM KCl 3) Actin monomers contain a molecule of ATP that is hydrolysed on polymerisation 4) Actin microfilaments run from the cell nucleus to the periphery of the cell 5) Microvilli are supported by microfilaments Question 5: The microtubules in a cell form