86H Pathogens and Pathogenesis Questions. 1 hour Workshop Block 1. Bacterial pathogens. Q1. From L3 paper on Std fimbriae. Describe in words what this genotype means. (see slide) The following 11 Qs are Data analysis and interpretation questions, and relate to Raffatellu et al paper, Fig2 and Fig 4. On the "workshop slides" (powerpoint file/pdf on VLE) these Qs are with the figures, which may also have some additional useful information. Q2. What is the experimental system for data shown in figure 2? And for Fig. 4? Fig 2 Q3 : what is the significance of showing the growth curves in B Q 4 why is data of experiments in C important to show? Fig. 4. Q5 : What is the relevance of streptomycin treatment? Q 6. what does each individual circle in A represent? Q7. What is key difference between experimental design and conclusions of A vs B? Q8. Where did I find the information on CI as used here (red)? Q 9. Why use Lcn2 -/- mice as in (B)? (Lcn = lipocalin) Q 10. Why use iroN in this CI experiment? Would iroB would give the same result? Q11: what does the last CI experiment (on the right) tell you about the role of iroN? Q12 : If in B a CI had been lower than 1 what would that mean? The 3 EXTRA Workshop Questions; assessment style GENERAL COMMENTS ON ASSESSMENTS · ANSWER THE QUESTION !! . NEEDS TO REFLECT YOUR KNOWLEDGE AND UNDERSTANDING . DO NOT ADD A LOT OF IRRELEVANT INFORMATION, THIS DOES NOT SHOW UNDERSTANDING We may not get around to discussing all of these. There is a separate file on VLE with some answer guidance/ hints. 1. You wish to carry out an experiment to test whether an E. coli strain adheres to patients' urothelial cells (epithelial cell layer of the bladder) using an animal model system. Your supervisor tells you to do another experiment first to address the 3R's. Describe one experimental approach you could take and why? 2. An anti-adhesive therapeutic for INTS infection is being developed which functions as a competitive inhibitor for Std fimbrial adhesion by competing with the fucose binding site. Outline advantages and disadvantages to developing such a therapy for iNTS disease.
3. A new bacterial strain has been isolated and is thought to be the causative agent of diarhea. You aim to carry out a competitive index (C.I.) experiment to help identify its mechanism of disease. You have an infection (animal) model that is relevant for the disease and pathogen, and measure CFU of specified mutants and WT in inoculum and colon content. You determine C.I. values using the equation: C.I. = (mutant/WT output ratio)/ (mutant/WT input ratio). . a) For one specific mutant you find a CI of 0.05. Is this consistent with a mutation in a toxin gene? Briefly justify your answer. b) You have one mutant that gives a CI of >1. What would your hypothesis be on the function or role