Lettuce (Lactuca sativa) is susceptible to a number of fungal pathogens including Botrytis cinerea. You carry out a mutagenesis screen and find fourteen individual plants that are resistant to infection. You perform an F1 complementation test. To do this you cross the mutants with each other and test the progeny for resistance (R) or susceptibility (S) to the fungus. The results of this analysis are given in the table below. Plant lines WT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 WT S S S S S S S S S S S S S S S 1 R S S S S S S S S S R S S S 2 R S S S S S S S R S S S S 3 R S S S S S R S S S R S 4 R S S S R S S S S S S 5 R S R S S S S S S R 6 R S S S S S R S S 7 R S S S S S S R 8 R S S S S S S 9 R S S S R S 10 R S S S S 11 R S S S 12 R S S 13 R S 14 R a) Why were all the mutant lines crossed to wild-type? To confirm that all the mutations were recessive b) How many complementation groups have been identified? Six d) How many alleles have been identified in each complementation group? 1, 11 (two), 2, 10 (two), 3, 9, 13 (three), 4, 8 (two), 5, 7, 14 (three), 6, 12 (two) e) How many genes have been identified that are required for the infection process of fungus? Six