BIO00018I Examination Candidate Number: Desk Number: University of York Department of Biology B. Sc Stage 2 Degree Examinations 2016-17 Postgenomic Biotechnology Time allowed: 1 hour and 30 minutes Total marks available for this paper: 80 This paper has three parts: Section A: Short answer questions (30 marks) · Answer all questions in the spaces provided on the examination paper Section B: Problem questions (20 marks) · Answer all questions in the spaces provided on the examination paper Section C: Long answer question (marked out of 100, weighted 30 marks) · Answer either question A or question B or question C . Write your answer on the separate paper provided and attach it to the back of the question paper using the treasury tag provided . The marks available for each question are indicated on the paper · A calculator will be provided For marker use only: 1 2 3 4 5 6 7 8 For office use only: Total as % page 1 of 9
BIO00018I SECTION A: Short answer questions Answer all questions in the spaces provided Mark total for this section: 30 1. Outline three characteristics of a polymerase used for PCR that could be improved by molecular engineering. (3 marks) 2. a) Explain when multiple displacement amplification (MDA) would be preferable to using the polymerase chain reaction (PCR) for amplifying a DNA sample? (1 mark) b) How do the reagents and conditions for MDA differ from those used in PCR? (6 marks) the space above this line should be sufficient for your answer page 2 of 9
BIO00018I 3. What by-products, released when nucleotide bases are incorporated during sequencing, are detected by pyrosequencing and ion semiconductor sequencing technologies respectively? (2 marks) 4. Which PCR phase is analysed in (quantitative PCR) qPCR and why? (2 marks) 5. Describe three quality control adjustments used in the analysis of two-channel microarray images (6 marks) the space above this line should be sufficient for your answer Turn over page 3 of 9