Question

A mixture of 15 amino acids gave the fingerprint shown here (see Problem 58 ). Identify the spots. (Hint $I:$ Pro reacts with ninhydrin to produce a yellow color; Phe and Tyr produce a green color. Hint 2: Count the number of spots before you start.)

Name: Problem 72, Chapter 21: Organic Chemistry
Uploaded: 2021-06-19T08:28:41-08:00
Duration: 2 min 38 s
Channel: Shahina -
Description: A mixture of 15 amino acids gave the fingerprint shown here (see Problem 58 ). Identify the spots. (Hint I: Pro reacts with ninhydrin to produce a yellow color; Phe and Tyr produce a green color. Hint 2: Count the number of spots before you start.)

   A mixture of 15 amino acids gave the fingerprint shown here (see Problem 58 ).
Identify the spots. (Hint $I:$ Pro reacts with ninhydrin to produce a yellow color; Phe and Tyr produce a green color. Hint 2: Count the number of spots before you start.)

Organic Chemistry

Paula Yurkanis… 8th Edition

Chapter 21, Problem 72

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Solved by Expert Shahina -

06/19/2021

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There are 15 spots, which corresponds to the 15 amino acids in the mixture. Show more…

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A mixture of 15 amino acids gave the fingerprint shown here (see Problem 58 ). Identify the spots. (Hint $I:$ Pro reacts with ninhydrin to produce a yellow color; Phe and Tyr produce a green color. Hint 2: Count the number of spots before you start.)

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Key Concepts

Chromatography

Chromatography is a technique used to separate and analyze components within a mixture based on differences in their movement through a stationary phase and interaction with a mobile phase. It is widely employed in analytical chemistry for identifying and quantifying compounds, including biomolecules like amino acids.

Amino Acid Analysis

Amino acid analysis involves the identification and quantification of amino acids present in a sample. This is a key process in biochemistry and molecular biology, providing insights into protein composition, structure, and function by separating amino acids and detecting their unique properties.

Ninhydrin Reaction

The ninhydrin reaction is a chemical test commonly used to detect amino acids due to its ability to produce colored compounds upon reacting with them. Different amino acids can yield distinct colored products when reacting with ninhydrin, which aids in their identification during chromatographic analysis.

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