00:01
The mixture of amino acids contain six of them, that is s -e -r -g -l -u -l -e -u, c -s -m -a -t -r -t -r, t -h -r.
00:47
In order to find out the identify the amino acids, we should know the structure of this amino acids so that we can distinguish between them.
01:02
Based on their polarity and charge existing at 5 ph so selling has a ch2 ohs site say glutamic acid c h 2 c .h2 c ox using c h g h c s3 c s t s t s t s t s t and he still has ch2 a heterosephac ring containing measured midazole double bond and h.
02:44
Then mitraline is ch2, ch2, s, ch3 while 309 is ch2, cxoh and ch.
03:16
So these are the structure.
03:18
Structure of this compounds.
03:22
After this, we can see that the histidine is basic.
03:48
At ph 5, this will be protonated.
03:59
This will be protonated and it will carry a positive chance.
04:06
Similarly, glutamic acid, glutamic acid, at h5 will have an overall negative charge.
04:31
Glutamic acid will be having an overall negative charge, ch2, ch2, coo minus at vh5.
04:47
So these two will be totally polar.
04:51
They are polar and in the electrophoresis, they will migrate this.
05:00
The histine will migrate to the cathode and glutamine acid to the anode.
05:19
This will migrate to the cathode and this will migrate to the anode...