An aqueous solution of 10.00 g of catalase, an enzyme found in the liver, has a volume of 1.00

step 1 Question starts off. 0 .745 tour. Let's change this to atmospheres. That will be equal to 9 .74

An aqueous solution of 10.00 g of catalase, an enzyme found...

An aqueous solution of $10.00 \mathrm{~g}$ of catalase, an enzyme found in the liver, has a volume of $1.00 \mathrm{~L}$ at $27^{\circ} \mathrm{C}$. The solution's osmotic pressure at $27^{\circ} \mathrm{C}$ is found to be $0.745$ torr. Calculate the molar mass of catalase.

Key Concepts

Colligative Properties

Colligative properties are properties of solutions that depend on the ratio of solute to solvent particles, rather than the identity of the solute. Osmotic pressure is one such property, and it is particularly useful for studying large molecules like proteins, because even very small concentrations of these macromolecules can produce measurable osmotic effects that can be analyzed to determine molecular characteristics such as molar mass.

Molar Mass Determination

Molar mass determination using colligative properties involves measuring a property such as osmotic pressure and using equations like ? = (n/V)RT to first calculate the number of moles of the solute present in a known volume. By relating the measured mass of the solute to the number of moles calculated, one can determine the molar mass of the solute. This method is particularly valuable for high molecular weight compounds such as proteins.

Osmotic Pressure

Osmotic pressure is a colligative property that represents the pressure required to stop the net flow of solvent molecules through a semipermeable membrane from a pure solvent into a solution. It depends on the number of solute particles in the solution and is typically expressed by the equation ? = cRT, where ? is the osmotic pressure, c is the molar concentration of the solute, R is the gas constant, and T is the absolute temperature.

van't Hoff Equation

The van't Hoff equation for osmotic pressure, given by ? = cRT, establishes a relationship between the osmotic pressure of a solution and its concentration, temperature, and the ideal gas constant. This equation is instrumental in determining properties such as the molar mass of solutes when the mass of dissolved substance and the solution volume are known, by allowing calculation of the molar concentration of the solute.

Please give Ace some feedback