00:02
Okay, so for this problem, what we're looking at is calculation of the isoelectric point.
00:09
So when we look at the pi of amino acid, we're basically kind of taking an average of the pkk that are relevant to their pronations taken.
00:23
So here we can see asparaging has two pkk, one of the carbideclic acid group at two, and one of the nitrogen group at just under 8.
00:37
To calculate their isoelectric point, all we're going to do is take the two pqas, add them together, and divide by two, since we have two pqas that we're taking into a account.
00:53
And this would give us a pi of roughly 5 .43.
01:02
We can do something very similar with serene.
01:06
Sarian again only has two pkk.
01:11
So if we were to take those two pqas, divide them by two again, because it only has two pkk that we are adding in.
01:23
And so let's just do some math real quick.
01:29
We have 2 .21 plus 9 .15 divided by 2 .6 .8, going to give us 5 .68, which is pretty typical for most of our sort of neutral or at least not charged residues.
01:54
So then we come to a problem like arginine.
01:57
Arginine has three functional groups that can be involved in protonation and deprotonation.
02:06
For argenine, we have that guanididium group at the very end that has three nitrogen surrounding a carbon...