Question
Describe and give the results of an experiment that shows that the G3-U70 base pair in a tRNA acceptor stem is a key determinant in the charging of the tRNA with alanine.
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This tRNA will serve as the control for the experiment. Show more…
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In a clever experiment performed in $1962,$ a cysteine already attached to its tRNA was chemically converted to an alanine. These "hybrid" tRNA molecules were then added to a cellfree translation system from which the normal cysteine-tRNAs had been removed. When the resulting protein was analyzed, it was found that alanine had been inserted at every point in the polypeptide chain where cysteine was supposed to be. Discuss what this experiment tells you about the role of aminoacyltRNA synthetases during the normal translation of the genetic code.
In a clever experiment performed in $1962,$ a cysteine already attached to its tRNA was chemically converted to an alanine. These "hybrid" tRNA molecules were then added to a cellfree translation system from which the normal cysteine-tRNAs had been removed. When the resulting protein was analyzed, it was found that alanine had been inserted at every point in the polypeptide chain where cysteine was supposed to be. Discuss what this experiment tells you about the role of aminoacyltRNA synthetases and ribosomes during the normal translation of the genetic code.
You are examining a mutant tRNA gene. For this mutant, assume that the mutant version is derived from an extra copy (i.e. all the wildtype tRNAs are still present). Additionally, assume that tRNA synthases are able to charge the tRNA with the stated amino acid. Determine what effect each mutant would have on translation. Use the wobble rules and translation table. A tRNA charged with Alanine (Ala) has an anticodon with the sequence 5' IGA 3':
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