Question
Describe and give the results of an experiment that shows the effects of CPSF and PAB II on polyadenylation of substrates with AAUAAA or AAGAAA motifs, with and without oligo( $A$ ) added. How do you interpret these results?
Step 1
- **PAB II (Poly(A) Binding Protein II)**: This protein binds to the poly(A) tail of mRNA, stabilizing it and enhancing its translation. - **AAUAAA and AAGAAA motifs**: These are signal sequences found near the 3' end of pre-mRNA which direct where cleavage Show more…
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