Question

Diagram the recombination repair mechanism in E. coli.

    Diagram the recombination repair mechanism in E. coli.

Molecular Biology

Molecular Biology

Robert F. Weaver 5th Edition

Chapter 20, Problem 33

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coli cell recognizes a damage in its DNA, such as a double-strand break (DSB). This can occur due to external factors like radiation or chemicals, or during replication errors. Show more…

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Diagram the recombination repair mechanism in E. coli.

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Key Concepts

Chi Sequence

A specific short DNA sequence in E. coli that modulates the activity of the RecBCD complex. Upon encountering the Chi sequence, RecBCD’s activity changes, reducing its nuclease function and promoting the proper loading of RecA onto the processed DNA, thereby directing the pathway towards homologous recombination.

Holliday Junction

A cross-shaped DNA structure that forms as an intermediate during homologous recombination. It represents the physical exchange of DNA strands between homologous molecules, and its resolution by specific enzymes is necessary to separate the intertwined DNA molecules and complete the repair process.

RecA Protein

A key protein in E. coli recombination repair that binds to single-stranded DNA to form a nucleoprotein filament. This filament is critical for searching for homologous sequences and promoting strand invasion, ultimately facilitating the exchange of genetic information during repair.

Homologous Recombination

A conserved DNA repair process that uses an undamaged homologous sequence as a template to repair damaged DNA. In E. coli, this mechanism is essential for repairing double-strand breaks and other complex DNA lesions by aligning and pairing the broken DNA strands with a complementary intact sequence.

RecBCD Complex

A multifunctional enzyme complex in E. coli that initiates homologous recombination repair by binding to and processing double-strand breaks. It unwinds and degrades the DNA until it encounters a specific regulatory sequence, helping to create a suitable substrate for RecA loading.

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