Question
Explique cómo apoyan los experimentos de microinyección la hipótesis de que una serie de moléculas específicas del citoplasma están implicadas en la transición de la interfase a la fase M. ¿ॄCuál fue el control para este experimento?
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Step 1: Los experimentos de microinyección implican la introducción de moléculas específicas en el citoplasma de una célula en interfase para observar su efecto en la transición a la fase M. Show more…
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Explain how microinjection experiments supported the hypothesis that specific molecules in the cytoplasm are involved in the transition from interphase to M phase. What was the control for this experiment?
A small amount of cytoplasm isolated from a mitotic cell is injected into an unfertilized frog oocyte, causing the oocyte to enter M phase (see Figure 18-7A). A sample of the injected oocyte's cytoplasm is then taken and injected into a second oocyte, causing this cell also to enter M phase. The process is repeated many times until, essentially, none of the original protein sample remains, and yet, cytoplasm taken from the last in the series of injected oocytes is still able to trigger entry into M phase with undiminished efficiency. Explain this remarkable observation.
A small amount of cytoplasm isolated from a mitotic cell is injected into an unfertilized frog oocyte, causing the oocyte to enter M phase (see Figure $18-7$ A). A sample of the injected oocyte's cytoplasm is then taken and injected into a second oocyte, causing this cell also to enter M phase. The process is repeated many times until, essentially, none of the original protein sample remains, and yet, cytoplasm taken from the last in the series of injected oocytes is still able to trigger entry into M phase with undiminished efficiency. Explain this remarkable observation.
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