Give the amino acid sequence of each peptide using the...

Name: Problem 59, Chapter 29: Organic Chemistry
Uploaded: 2020-03-02T10:43:25-08:00
Duration: 5 min 36 s
Channel: Anupa Sharad Medhekar
Description: Give the amino acid sequence of each peptide using the fragments obtained by partial hydrolysis of the peptide with acid. a. a tetrapeptide that contains Ala, Gly, His, and Tyr, which is hydrolyzed to the dipeptides His-Tyr, Gly-Ala, and Ala-His b. a pentapeptide that contains Glu, Gly, His, Lys, and Phe, which is hydrolyzed to His-Gly-Glu, Gly-Glu-Phe, and Lys-His

Give the amino acid sequence of each peptide using the fragments obtained by partial hydrolysis of the peptide with acid. a. a tetrapeptide that contains Ala, Gly, His, and Tyr, which is hydrolyzed to the dipeptides His-Tyr, Gly-Ala, and Ala-His b. a pentapeptide that contains Glu, Gly, His, Lys, and Phe, which is hydrolyzed to His-Gly-Glu, Gly-Glu-Phe, and Lys-His

Give the amino acid sequence of each peptide using the fragments obtained by partial hydrolysis of the peptide with acid.
a. a tetrapeptide that contains Ala, Gly, His, and Tyr, which is hydrolyzed to the dipeptides His-Tyr, Gly-Ala, and Ala-His
b. a pentapeptide that contains Glu, Gly, His, Lys, and Phe, which is hydrolyzed to His-Gly-Glu, Gly-Glu-Phe, and Lys-His

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Key Concepts

Peptide Bond Cleavage

Peptide bonds are the chemical links between amino acids in a peptide. Understanding which bonds are cleaved during hydrolysis, and how these cleavages generate specific overlapping fragments, is key to determining the primary structure of the peptide.

Overlapping Fragment Analysis

This method involves reconstructing the complete peptide sequence by analyzing overlaps among the fragments generated by partial hydrolysis. Each fragment shares common amino acids with neighboring fragments, allowing researchers to deduce the correct order of residues in the original peptide through logical sequence alignment.

Partial Hydrolysis

Partial hydrolysis is a controlled chemical process where peptide bonds in a protein are selectively cleaved under acidic conditions. This method does not completely break down the protein into individual amino acids but rather produces overlapping peptide fragments, which can be analyzed to infer the original sequence.

Amino Acid Sequence

This concept refers to the specific order in which amino acids are linked together by peptide bonds to form a peptide or protein. Understanding the amino acid sequence is fundamental because it determines the protein's structure and function, and is central to techniques in molecular biology and biochemistry for determining protein identity and properties.

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