00:01
All right, so we see in this problem that it gives us hemoglobin, which is the protein responsible for carrying oxygen through blood, has an isoelectric point of 6 .8, right? so it's asking us, what is the net charge at these two phs? so at ph 5 .3, right? we've got that the ph is lower than the pi.
00:26
So what that means is that you essentially, you can think of, since the ph is lower, or that means there's more h plus, right? so you can think of having an excess of h plus.
00:43
So what that means is that you're probably going to be protonating the free carbonyxylic acid group and as well as the mean.
00:52
So you're going to have, if i have just, you're going to have that h3n plus and that c -o -o -h.
01:03
So because of that, i can say that the net charge is going to be positive.
01:10
So now on the other hand, we can think of that the ph 7 .8, so ph is greater than the p .i.
01:20
So you can think of this of having pretty much the opposite kind of argument, that there's going to be less acid or more base...