Question
How much nitrogen is present in a $250 \mathrm{~mL}$ solution containing $10 \%$ of protein $\mathrm{X}$ and $5 \%$ of protein $\mathrm{Y}$.
Step 1
Given that the solution contains 10% of protein X, we can calculate the amount of protein X in the solution as follows: Amount of protein X = 10% of 250 mL = 0.10 * 250 mL = 25 mL Show more…
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The nitrogen percentage of a sample containing 37.9 mg protein in 1 mL is analyzed by the Kjeldahl Method. (It is a special method, you can find it on Google if you don't know.) For this, 0.256 mL of the sample solution is taken and the NH3 released as a result of the processes is collected in 5.00 mL of 0.0336 M HCl solution. The HCl solution is titrated with 6.34 mL of 0.010 M NaOH. Calculate the percentage by mass of nitrogen in the protein.
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