Human DNA and a particular plasmid both have sites that are...

Name: Problem 3, Chapter 14: Biology
Uploaded: 2022-01-31T06:46:56-08:00
Duration: 2 min 44 s
Channel: Sirisa Reddy
Description: Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) use HindIII to cut both the plasmid and the human DNA (d) a or b (e) b or c.

Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) use HindIII to cut both the plasmid and the human DNA (d) a or b (e) b or c.

Labs

Want to see this concept in action?

NEW

Explore this concept interactively to see how it behaves as you change inputs.

View Labs

Key Concepts

Sticky Ends

Sticky ends refer to the single-stranded overhangs produced when DNA is cut by certain restriction enzymes. These overhangs allow DNA fragments with complementary sequences to anneal, facilitating the joining (ligating) of different DNA molecules in recombinant DNA technology.

Fragment Compatibility

For successful recombinant DNA construction, the DNA fragments must have compatible ends. This means both the vector and the insert should be cut with the same restriction enzyme to produce matching sticky ends, ensuring that they can be efficiently ligated together.

Recombinant DNA

Recombinant DNA is the process of combining DNA fragments from different sources to create new genetic combinations. It requires the precise joining of DNA segments, where compatibility between the ends of these fragments is essential for successful ligation.

Restriction Enzymes

Restriction enzymes are proteins that recognize specific DNA sequences and cut the DNA at or near these sites. They are fundamental tools in molecular biology as they generate fragments with defined ends, which can be used for cloning and other genetic manipulations.

Transcript

Please give Ace some feedback