Match the recombinant DNA method with the appropriate enzyme. PCR cutting DNA cDNA synthesis DNA

Match the recombinant DNA method with the appropriate...

Key Concepts

DNA Sequencing

DNA sequencing encompasses methods used to determine the exact order of nucleotides within a DNA molecule. Modern sequencing techniques often involve DNA polymerases to incorporate nucleotides in a controlled manner, facilitating the reading of the DNA code.

PCR

PCR, or Polymerase Chain Reaction, is a technique used to amplify small DNA segments into millions of copies. This method uses thermal cycling along with DNA polymerases, commonly thermostable ones like Taq polymerase, to replicate the target DNA through repeated cycles of denaturation, annealing, and extension.

Restriction Enzymes

Restriction enzymes are proteins that cleave DNA at specific sequences, often used in molecular cloning and recombinant DNA technologies. They create breaks at defined locations in the DNA, which can be used to cut and recombine DNA fragments for various applications.

Reverse Transcriptase

Reverse transcriptase is an enzyme that synthesizes complementary DNA (cDNA) from an RNA template. This process is essential in cDNA synthesis and is widely used in research involving gene expression analysis, cloning, and RNA virus replication.

DNA Ligase

DNA ligase is an enzyme responsible for joining DNA fragments together by forming phosphodiester bonds between the 3'-hydroxyl and 5'-phosphate ends. This 'pasting' process is crucial in DNA repair and recombinant DNA assembly during cloning processes.

Transcript

00:01 So the first option for matching in terms of the recombinant dna method with its enzyme, its associated enzyme, is pcr, polymerase chain reaction.

00:12 And since polymerase in the name, you would be correct to assume that one of the correct answers is either tac polymerase or dna polymerase, because they are both essential enzymes involved in dna synthesis.

00:23 And so tacpolymerase is taken from an archaille organism.

00:31 That is thermophilic and thrives in high temperatures.

00:36 And this would be the correct answer because if you remember from pcr, it occurs in cycles in which at some stages the reaction is heated to a very high temperature that a standard mammalian dna preliminaries would not be able to withstand it with denature and it would be rendered and effective.

00:52 So for pcr, the answer would be a tap polymerase.

00:55 For cutting dna, the second option, you are going to be looking for an enzyme.

01:01 That is going to be able to recognize a site within the dna and cut at that site.

01:08 And that is the job of a restriction enzymes.

01:11 Restriction enzymes all have complementary dna sequences that they search the dna for.

01:18 And when they find that complementary site, they cut.

01:21 And they will split the dna.

01:22 There are two methods in which it will split in kind of a blunt -ended dna segment or with a little bit of a overhang, which is known commonly as, sticky ends.

01:33 And so the answer for cutting dna would be a restriction enzyme, and that is letter d.

01:39 For c dna synthesis, that is complementary dna synthesis.

01:43 You are synthesizing the complementary strand of dna to the strand that you are looking at.

01:49 And so that would be reverse transcript days because you are doing complementary strand synthesis.

01:56 You are not just synthesizing the same strand of dna.

01:59 If you were synthesizing the same strand of dna, you would be using dna polymerase...

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