00:01
So, in this question, d value will represent the time required to achieve 90 percent reduction that is one cycle reduction.
00:15
And then the z value here will represent the temperature range required to achieve ten fold change in the d value.
00:24
So, d value is at 85 degrees celsius.
00:28
We're looking at 8 .3 minutes.
00:32
And z value we're looking at 14 degrees celsius.
00:36
Desired reduction in enzyme activity, we're looking at 99 percent.
00:41
That is 0 .99 reduction or let me say two log cycles.
00:49
You can't see that.
00:51
So, we need to calculate the time required to achieve these two log cycles first.
00:54
So, t will be equal to d value.
01:00
Multiply by log of 10 of desired reduction.
01:04
So, we're looking at 8 .3 minutes.
01:09
Multiply by log of 10 into 0 .01...