00:01
Today we're discussing lab techniques and that's going to help us answer questions 15 to 19 later on.
00:10
So starting with gel electrophoresis.
00:13
That's actually the setup that i have right here.
00:18
And gel electrophoresis is a lab technique that involves submerging a gel in a buffer.
00:28
So this whole square thing right here is the gel and the blue liquid is our buffer.
00:39
This whole setup is ran in an electric field.
00:45
So this is our positive end and the negative end of our electric field.
00:51
And we do this because we want to separate dna fragments.
00:56
Dna fragments.
01:01
So these fragments of dna get separated as they go down the gel.
01:05
So after this experiment, we're going to see little fragments of dna stuck to the gel.
01:11
And that tells us information about their size.
01:15
Because as they go down, only the small dna fragments can continue down.
01:22
Moving on to restriction enzymes.
01:26
Restriction enzymes are used to cut dna, cut dna, and we use it a lot with genetic engineering.
01:36
Genetic engineering.
01:38
When we want to remove a part of dna and replace it with something else.
01:44
So it's very helpful for those types of research.
01:47
We use it a lot for cloning as well, so restriction enzyme.
01:51
Next we have polymerase chain reaction, or pcr.
01:59
And as i've mentioned in a previous video, pcr is used to amplify dna.
02:08
Amplify dna.
02:09
And the word amplify here really just means to make more of it.
02:13
So we're just making more of dna...