Question
Restriction enzymes of $\mathrm{E}$. coli are(a) HindIII(b) BamHI(c) EcoRI, EcoRII(d) All of these
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Restriction enzymes are endonucleases produced by bacteria. These restriction enzymes: a) Typically recognise specific 4 to 8 bp sequences called restriction sites b) Cleave both strands of DNA at their specific restriction sites c) Ligate cut DNA by re-establishing phosphodiester linkages d) a, b and c above e) a and b above f) None of the above
Enzymes used to cut genes in recombinant DNA research are... a. ligases b. restriction enzymes c. transcriptases d. DNA polymerases e. replicases
Restriction enzymes $a$ play no role in bacteria. $b$. cleave DNA at highly specific recognition sequences. $c .$ are inserted into bacteria by bacteriophages. $d$. are made only by eukaryotic cells. $e .$ add methyl groups to specific DNA sequences.
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