Question
Reverse transcriptase can help make genes for cloning. cDNA can be used to identify the genes that are being transcribed by a particular cell at a given moment.
Step 1
This is crucial because mRNA represents the actively transcribed genes at a particular moment. Use techniques such as using oligo(dT) magnetic beads which bind to the poly-A tail of eukaryotic mRNA. Show more…
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Reverse transcription PCR is used specifically to perform PCR at cooler temperatures. detect the presence of RNA viruses. detect the presence of gene expression in a given cell. measure how many copies of a specific gene are in a sample. replace a mutated gene with a corrected gene.
$\mathrm{PCR}$ can be used _____________ . a. as a cloning vector b. in DNA profiling c. to modify a human genome
A DNA gene synthesized from an RNA template is ________ . a. reverse transcriptase b. complementary DNA c. recombinant DNA d. probe DNA
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