Transposon Tn T in plasmid A transposes to plasmid B. How...

Transposon $\mathrm{Tn} \mathrm{T}$ in plasmid A transposes to plasmid B. How many copies of $\mathrm{TnT}$ are in the cointegrate? Where are they with respect to the two plasmids in the cointegrate?

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Key Concepts

Replicative Transposition

This is a mechanism by which a transposable element, or transposon, duplicates itself during the process of moving from one DNA molecule to another. Instead of simply cutting itself out from one location and pasting into another, the transposon creates a copy of itself during transposition, ensuring that the original copy remains intact. This leads to a duplication event, resulting in two copies of the transposon, one remaining in the donor molecule and the other integrating into the target molecule.

Cointegrate Formation

Cointegrate formation is a process where two separate DNA molecules, such as plasmids, become fused into one larger molecule during replicative transposition. In the cointegrate, the transposon is present in duplicate, with one copy situated at each junction between the two original plasmids. This configuration results from the replication of the transposon during the transposition event, which effectively links the donor and target molecules together.

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