Question

Using a cDNA library, you isolated two different cDNA clones that have sequences indicating that they both correspond to mRNAs transcribed from the same nerve growth factor gene. The beginning and ending sequences of the clones are the same, but the middle sequence is different. How can you explain the different cDNAs?

Name: Problem 13, Chapter 10: Genetics: From Genes to Genomes
Uploaded: 2023-01-15T09:45:41-08:00
Duration: 1 min 58 s
Channel: Jennifer Stoner
Description: Using a cDNA library, you isolated two different cDNA clones that have sequences indicating that they both correspond to mRNAs transcribed from the same nerve growth factor gene. The beginning and ending sequences of the clones are the same, but the middle sequence is different. How can you explain the different cDNAs?

   Using a cDNA library, you isolated two different cDNA clones that have sequences indicating that they both correspond to mRNAs transcribed from the same nerve growth factor gene. The beginning and ending sequences of the clones are the same, but the middle sequence is different. How can you explain the different cDNAs?

Genetics: From Genes to Genomes

Leland Hartwell,… 6th Edition

Chapter 10, Problem 13

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Solved by Expert Jennifer Stoner

01/15/2023

Step 1

A cDNA library is a collection of cloned cDNA (complementary DNA) fragments, which is used to express the proteins of an organism in a different host or to clone and analyze genes. Show more…

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Using a cDNA library, you isolated two different cDNA clones that have sequences indicating that they both correspond to mRNAs transcribed from the same nerve growth factor gene. The beginning and ending sequences of the clones are the same, but the middle sequence is different. How can you explain the different cDNAs?

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Key Concepts

cDNA Library

A cDNA library is a collection of cloned complementary DNA (cDNA) fragments, each derived from mRNA molecules. Since cDNA is synthesized from mature mRNA, it represents only the expressed parts of the genome (exons), providing a snapshot of gene expression in a particular cell type or condition. This tool is crucial for studying the products of genes and their functional variants, such as those produced by alternative splicing.

Alternative Splicing

Alternative splicing is a post-transcriptional process where different exons of a pre-mRNA transcript are joined in various combinations, resulting in multiple distinct mRNA molecules from a single gene. This mechanism allows cells to produce different protein variants with potentially diverse functions from the same genetic sequence, significantly increasing the functional complexity of the genome.

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