00:01
This question asks, predict the effect of an alteration in the sequence of nucleotides in the negative 35 region of a bacterial promoter.
00:10
So here we have a diagram illustrating the bacterial promoter region, the operator region, the overall operon, and a upstream regulatory gene sequence.
00:23
And we will basically use this diagram to understand how an alteration in the negative 35 region of the motor will have an effect on the overall transcription of our genes that we need to transcribe.
00:39
So if we think about it, the negative 35 region is important because it is going to be where the sigma factor will bind.
01:08
So if we have a different nucleotide there, the sigma factor might not be able to recognize where it has to bind.
01:18
So an alteration to this negative 35 region can prohibit sigma factor binding, which becomes a problem because the sigma factor is needed in order to be able to assemble all the necessary framework and machinery to begin transcription...