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Hello everyone.
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Today we're going to discuss about whole genome shotgun method or simply shotgun method.
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Now what is this shotgun method? shortgun method is actually the most efficient way to sequence large pieces of dna.
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It is the best method for the sequencing of genome.
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Now, let's see about its procedure.
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What actually happens here? in this method, first of all, we have dnaps broken down into small fragments, just like short -win fashion.
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Then each of the fragment undergoes sequencing.
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After the sequencing of each of the fragment is done individually.
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The next step is analysis of different pieces of fragments by computer.
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This analysis involves looking up for stretches.
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Stretches are the terminal portions of these small fragments.
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In the next step, identical stretches or the fragments having identical stretches are brought closer to each other.
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When the identical regions are identified, they're overlapped with each other.
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There is overlapping of fragments with identical stretches...