Question
When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?
Step 1
These enzymes cut the DNA at specific sequences, resulting in linear pieces of DNA. This can be represented as: \[ \text{DNA} \xrightarrow{\text{Restriction Enzymes}} \text{Linear DNA pieces} \] Show more…
Show all steps
Your feedback will help us improve your experience
Sam Limsuwannarot and 63 other Organic Chemistry educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Why is the density of the gel important in gel electrophoresis? Prevent DNA contamination Protect DNA from digestion Cause DNA denaturation Avoid DNA replication Influence the rate of migration
Explain how gel electrophoresis is used to separate DNA frag ments of different lengths.
Explain how gel electrophoresis is used to separate DNA fragments of different lengths.
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD