Question

Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process? a. DNA polymerase b. RNA polymerase c. A restriction enzyme d. DNA ligase e. An aminoacyl-tRNA synthetase f. Peptidyl transferase g. Reverse transcriptase

Name: Problem 4, Chapter 10: Genetics: From Genes to Genomes
Uploaded: 2023-04-20T17:04:24-08:00
Duration: 3 min 7 s
Channel: Jenny Wu
Description: Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process? a. DNA polymerase b. RNA polymerase c. A restriction enzyme d. DNA ligase e. An aminoacyl-tRNA synthetase f. Peptidyl transferase g. Reverse transcriptase

   Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process?
a. DNA polymerase
b. RNA polymerase
c. A restriction enzyme
d. DNA ligase
e. An aminoacyl-tRNA synthetase
f. Peptidyl transferase
g. Reverse transcriptase

Genetics: From Genes to Genomes

Leland Hartwell,… 6th Edition

Chapter 10, Problem 4

Instant Answer

Solved by Expert Jenny Wu

04/20/2023

Step 1

This is done because mRNA carries the genetic information from the DNA to the ribosome, the place of protein synthesis in the cell. Show more…

Show all steps

Thanks for your feedback!

Which of the enzymes from the following list would you need to make a cDNA library? What is the function of those enzyme(s) in the process? a. DNA polymerase b. RNA polymerase c. A restriction enzyme d. DNA ligase e. An aminoacyl-tRNA synthetase f. Peptidyl transferase g. Reverse transcriptase

Play audio

Feedback

Jenny Wu and 52 other subject Biology educators are ready to help you.

Ask a new question

Labs

Want to see this concept in action?

NEW

Explore this concept interactively to see how it behaves as you change inputs.

View Labs

Key Concepts

Reverse Transcriptase

Reverse transcriptase is an enzyme that synthesizes DNA using an RNA template, a process known as reverse transcription. In creating a cDNA library, reverse transcriptase converts mRNA into its complementary DNA (cDNA), which can then be cloned into vectors for further analysis. This conversion is crucial, as it transforms the relatively unstable RNA into a more durable DNA form that accurately reflects the expressed genes.

cDNA Library

A cDNA library is a collection of complementary DNA sequences synthesized from the messenger RNA (mRNA) of a cell or tissue. It represents only the coding regions of the genome, reflecting the genes that are actively being expressed. This library is useful for gene cloning, expression studies, and functional analysis because it excludes non-coding regions like introns.

Please give Ace some feedback