Which of the following steps are catalysed by Taq polymerase...

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
(a) Denaturation of template DNA
(b) Annealing of primers to template DNA
(c) Extension of primer end on the template DNA
(d) All the above

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Key Concepts

Taq Polymerase

Taq polymerase is a thermostable DNA polymerase isolated from Thermus aquaticus that retains activity at high temperatures. It is used primarily in the extension step of PCR, where it catalyzes the polymerization of nucleotides to synthesize new DNA strands, rather than being involved in denaturation or primer annealing.

PCR Process

The Polymerase Chain Reaction (PCR) is a method used to amplify DNA through repeated cycles that include distinct steps: denaturation, where the DNA double helix is separated by high temperature; annealing, where primers bind to their complementary sequences on the single-stranded DNA; and extension, where Taq polymerase synthesizes new DNA strands by adding nucleotides.

Enzymatic Catalysis in PCR

Enzymatic catalysis in PCR refers to the role of enzymes such as Taq polymerase in mediating biochemical reactions, specifically the addition of nucleotides during the extension phase. Although the PCR process includes steps driven by temperature changes like denaturation and annealing, the enzyme's catalytic activity is confined to facilitating the chemical reaction that forms new DNA strands.

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